Figure 1.

Molecular analysis of the t(14;21) breakpoints. (A) Restriction map of the germ-line (GL) and derivative (DER) alleles. Cen, centromere; tel, telomere; locations of the Jα70, 0.4NN, and 0.7 HR probes are indicated. B, BamHI; H, HindIII; R, EcoRI; X, XbaI. (B) Nucleotide sequence at breakpoint. The germ-line Vα sequence was obtained from GenBank (HUAE000660). The germ-line (GL) and derivative (DER) alleles are presented. Jα and Vα heptamer sequences are underlined, a potential translin binding site is shown in bold. Nontemplated nucleotides from the DER14 are shown in lowercase.