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In vitro transcriptional repression by RheA. Purified RheA was assayed for its ability to repress run-off transcription of the hsp18 and rheA genes at various temperatures in the presence of the S. coelicolor RNA polymerase. The molecular weight markers (in nucleotides) correspond to HpaII-digested pUC19. Transcripts of the expected size, 147 and 201 nt, were observed for Phsp18 and PrheA, respectively.
