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Conformational change analyzed by CD spectroscopy. CD spectra of the purified RheA (5 μM). The spectra were recorded in the same cell at 25°C (—), 30°C (- —), 35°C (– –), 41°C (⋅ ⋅ ⋅), and 43°C (- —) and after a temperature shift-back to 30°C (- - -).
