Figure 3.

Photomicrographs showing immunolabeling for GAD₆₇ (A), Dab1 (B), Reln (C), and integrin α₃ subunit in the patas PFC. (A) Immunostaining for GAD₆₇ reveals a GAD₆₇-positive GABAergic cell (solid arrow) as well as numerous punctate GAD₆₇-positive terminal endings in the neuropil and surrounding excitatory pyramidal cells (open arrows) of layer V. (B) Immunostaining for Dab1 in layer V shows that it is expressed in excitatory pyramidal cells. (C) Immunostaining for Reln reveals cells that appear to contain Reln, such as the GABAergic bitufted cell indicated with a solid arrow in layer III, as well as many pyramidal cells that appear to have a light dusting of Reln (open arrows). (D) Photomicrograph of layer V showing that the integrin α₃ subunit appears to be well expressed on pyramidal cells and their apical dendrites. (Calibration bar = 20 μm.)