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. 2000 Mar 21;97(7):3556–3561. doi: 10.1073/pnas.050589597

Figure 2.

Figure 2

Linearity of Western blot chemifluorescence intensity of serial dilutions of Reln H-protein standard (●) and 400-kDa Reln-LI protein (○) obtained from CGN extracts probed with the G-10 antibody. Experimental conditions are as described in Fig. 1. Given the linear range of assay for Reln H-protein, between 5 and 40 fmol, and considering that the volume applied to the gel was 40 μl, the final concentration of Reln H-protein standard solution was between 125 and 1,000 pM. Thus, the volume of the cell extract was adjusted to obtain similar Reln concentrations.