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Immunocytochemistry of fetal rat-brain neurons. (A–C) Results of a 3-day control culture. (D–F) PREG (1 μM) added the last 2 days of culture. The antibodies used were monoclonal anti-α-tubulin antibody (A and D), polyclonal anti-doublecortin antibody (B and E), and monoclonal anti-MAP2 antibody (C and F). PREG increased the immunostaining of cell bodies and induced the appearance of immunostaining in proximal neurites (arrows). (Scale bar = 0.2 nm.)
