Figure 2.

Efficiency of channel formation determined by time course and RNA dose response of ShB, ShBΔ96–183, and ShBΔ96–183_GCN4LI. Oocytes were injected with concentrations of RNA as indicated. Peak currents resulting from a depolarization from −80 mV to + 30 mV at different times after injection are plotted in graph. Data points reflect mean and SE for 3–5 oocytes. Leak-subtracted currents from two electrode voltage-clamp recordings were elicited by 100-ms depolarizing steps from a holding potential of −80 mV to −30, −10, 10, 30, and 50 mV in 2 mM K⁺.