Figure 1.

ErbB receptors are present in the post synaptic density fraction. The subcellular distribution of ErbB receptors in rat forebrain fractions enriched in synaptic membranes (SM) and postsynaptic densities (PSD) was analyzed by Western blot. The blots were probed with specific antisera raised against ErbB-2, ErbB-3, and ErbB-4, and the postsynaptic proteins PSD-95 and NR1. Antisera against synaptophysin (SynP) and glutamate transporter-1 (GLT-1) were used to monitor the presence of presynaptic and glial markers, respectively, in the SM and PSD fractions. All lanes contain 37 μg of protein.