Figure 2.

Coimmunoprecipitation of ErbB-4 receptor and PSD-95 from forebrain lysates. (A) Total proteins from rat forebrain deoxycholate homogenates (Western, Left), or proteins immunoprecipitated from these lysates (IP, Right), were resolved by SDS/PAGE and probed on Western blots with ErbB-4 (E) and PSD-95 (P) antisera. The antibodies specifically recognize proteins of approximately 180 kDa (small arrowhead) and 95 kDa (large arrowhead), respectively. Migration of the prestained molecular weight markers (myosin, β-galactosidase, and BSA) are indicated. (B) Coimmunoprecipitations were performed from forebrain deoxycholate lysates using PSD-95 (Left) or ErbB-4 (Right) antibodies, and probed on Western blots using anti-ErbB4 or anti-PSD-95 antisera. As negative controls, the immunoprecipitations were performed either in the absence of antibodies (−), normal rabbit immunoglobulins (IgG), or monoclonal antibodies raised against the unrelated transcription factor E2F1 (an IgG2a that is the same isotype as the PSD-95 monoclonal).