Figure 3.

Effects of tyrosine kinase inhibitors on E2-mediated protection of the C-terminal domain of GluR1 or NR2 subunits against NMDA-elicited truncation. Hippocampal slices were treated with NMDA (50 μM) for 3 h. E2 and PP2 or PD98059 were added as described for Fig. 2. Immunoblots were performed as described in Materials and Methods and were labeled with antibodies against the C-terminal domains of GluR1 (105 kDa) or NR2 (170 kDa) subunits. (A) Representative blot, with the order of treatment as indicated in B. (B) Quantification of blots similar to the ones shown in A. Results are expressed as the percentage of values found in control conditions and are means ± SEM of six to eight experiments.