Figure 5.

Effects of an src inhibitor on E2-mediated enhancement of EPSP amplitude and degree of LTP in acute hippocampal slices. Acute hippocampal slices were prepared as described in Materials and Methods. A stimulating electrode was located in CA3, and a recording electrode was located in stratum radiatum of CA1. Extracellular EPSPs were evoked by stimulation every 30 s, and the amplitude of the EPSP was measured. After a stable baseline was recorded, PP2 (10 μM) was added at the indicated time. Likewise, E2 (1 nM) was added at the indicated time. After resetting the stimulation intensity to obtain an EPSP of the same amplitude as before treatment with PP2 or E2, high frequency stimulation was delivered, and low frequency stimulation was resumed. Results are expressed as percentage of predrug values and are means ± SEM of 6–10 experiments. HFS, high-frequency stimulation.