Figure 5.

PKC-dependent modulation of sIPSCs is prevented by 3α-OH-DHP during late pregnancy, but not after parturition. (a and b) TPA (25 nM) induced a significant (**, P < 0.01) suppression in the absence of 3α-OH-DHP, but not after pretreatment with 3α-OH-DHP (1–10 μM) in P20 stage animals. (c and d) At PPD1, the effect of TPA was observed regardless of the presence of 3α-OH-DHP (** and *, P < 0.01 and 0.02, respectively). Note that 3α-OH-DHP also fails to affect the decay of the IPSCs at this stage. Traces in a and c are averages of a minimum of 50 individual sIPSCs per condition. All tests summarized in b and d are from n = 4 recordings from n = 4 animals.