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Effects of E2 on endothelial cell proliferation. (a) The effects of E2 (10−8 M) and l-NAME (1 mM) on population doublings in each passage of HUVECs. The treatment time with E2 or l-NAME was 24 h. ∗, P < 0.05 vs. control. (b) The effects of different concentrations of E2 on relative cell division of HUVECs. Cells were used in PDL 20.2 at passage 7. ∗, P < 0.05: cell division vs. control.
