Figure 3.

Overexpression of STC increases cell resistance to hypoxic insult. (A) Paju cells transfected with stc cDNA (Paju/STC) expressed STC as shown by Western blotting. (B) Morphology of control Paju/C (Paju cells transfected with empty vector) and Paju/STC cells after 24 h in the presence of 300 μM CoCl₂. (C) Cell viability assay of Paju/C and Paju/STC cells treated with CoCl₂ for indicated time periods. (D) ATP contents of Paju/C and Paju/STC cells treated with 300 μM CoCl₂ for indicated times. The data are presented as mean ± SD. *, P < 0.05 compared with control samples (Student's t test, n = 5–6).