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Response of aortic rings taken from normal rats to a time-dependent loss of phenylephrine (PE)-induced tone. PE was added as indicated by the arrow after 1.5 h equilibration and then rings were treated with either (a) polymyxin B (10 μg ml−1) (b) doxycycline (30 μM) or ddH2O vehicle or (c) 10 μM GM6001 or 0.1% ethanol vehicle (*P<0.05, two-way repeated measures ANOVA, n=4–5 aortic rings taken from distinct animals/group).
