Figure 2.

Effect of PAR-2AP administration on GSSG and reduced GSH as indexes of oxidative reperfusion-injury. At 20 min of stabilization, GSSG and GSH were not detected. PAR-2AP dose dependently (10–100 μM) reduced GSSG and GSH levels in effluents from isolated rat heart after 20 min of ischemia and at 60 min of the reperfusion period. Genistein did not revert PAR-2AP effect. Trypsin caused a small but significant inhibition of GSSG only at 60 min of the reperfusion period. Data are expressed as mean ± SD; n = 6; *, P < 0.05 vs. control and scrambled peptide.