Figure 6.

Effect of the inhibition of endocytosis on BRET. BRET ratio was measured in cells expressing the indicated constructs in the presence or absence of the β₂-adrenergic and CCR5 agonists isoproterenol (10 μM) and MIP-1α (0.2 μM). The effect of coexpressing the dominant negative mutant of dynamin (DynK44A) was also assessed. The data represent the mean ± SEM of four independent readings. Receptor expression levels were as follows: β₂AR-Rluc, 211 fmol/mg protein; β₂AR-Rluc and β₂AR-YFP, a total of 398 fmol/mg protein; β₂AR-Rluc and CCR5-YFP, 400 fmol/mg protein for the β₂AR and 456 fmol/mg protein for CCR5.