Figure 4.

1-Octanol antagonizes ethanol inhibition of BMP-7 morphogenesis. NG108–15 cells were cultured for 3 days in serum-free medium supplemented with the indicated concentrations of BMP-7, ethanol, and 1-octanol. (A–D) Photomicrographs were obtained under phase-contrast microscopy (×200 magnification) from cells treated as follows: (A) no additions (control); (B) 10 ng/ml BMP-7; (C) 10 ng/ml BMP-7 and 50 mM ethanol; (D) 10 ng/ml BMP-7, 50 mM ethanol, and 0.05 mM 1-octanol. (E) NG108–15 cells were cultured for 3 days in the presence of 0.1–40 ng/ml BMP-7. Parallel cultures were treated with 50 mM ethanol or 50 mM ethanol plus 0.05 mM 1-octanol. The percentage of cells in clusters of three or more cells was scored from two separate fields viewed under ×200 magnification. Shown are the mean ± SEM for the percentage of cell clusters from three to four independent experiments. (Scale bar = 100 μm.)