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Analysis of [3H]estradiol binding in the PCY2 yeast. (A) Saturation binding. (B) Scatchard analysis. The Kd value was 3.00 ± 0.24 nM and Bmax was 9.39 ± 3.5 fmol per million cells. Both figures are representative of three separate experiments with each concentration of [3H]estradiol run in quadruplicate.
