Table 1.
Summary of the effects of E2, tamoxifen, and ICI 182,780 on the hER in yeast genetic systems
| Treatment | ER/62/ERE yeast
|
||||||
|---|---|---|---|---|---|---|---|
| Two-hybrid yeast
|
No CuSO4
|
+ 100 μM CuSO4
|
|||||
| EC50, nM | β-gal max, RRU | Ki, nM | EC50, nM | β-gal max, RRU | EC50, nM | β-gal max, RRU | |
| E2 | 0.16 (0.02) | 43.8 (7.5) | 3.47 (0.12) | 1.23 (0.23) | 9.03 (1.30) | 1.18 (0.18) | 178.2 (13.7) |
| Tamoxifen | 1,536 (830) | 23.0 (2.6) | 2,610 (190) | >30,000 | ND | >30,000 | ND |
| ICI 182,780 | 2,355 (233) | 44.6 (6.6) | 5,230 (1,240) | 806 (320) | 7.92 (1.23) | 3,400 (250) | 222.4 (5.8) |
Values are means (SEM) from three or four separate experiments. ND, not determined.