Table 2.
Effect of anti-estrogens on E2 dose response in ER/62/ERE yeast
| E2 | + 10 μM TMX | + ICI
|
|||
|---|---|---|---|---|---|
| 10.0 μM | 3.0 μM | 1.0 μM | 0.3 μM | ||
| EC50 (nM) | 1.15 | ND | 1.76 | 0.51 | 0.52 |
| (0.12) | (0.31) | (0.20) | (0.11) | ||
| β-gal max (RRU) | 7.86 | 9.76 | 10.52* | 13.34* | 16.50* |
| (0.43) | (2.90) | (0.04) | (1.04) | (2.00) | |
Values are means (SEM) from three or four separate experiments. Each concentration of the dose response curves was run in triplicate. The ER/62/ERE yeast used in the E2 dose response studies with added antiestrogen were grown in the absence of CuSO4. TMX, tamoxifen; ND, not determined; *, P < 0.5 vs. E2 only.