Table 3.

[³H]Estradiol binding (cpm) in the two-hybrid yeast in the presence and absence of the hER

[3H]Estradiol, nM	Absence of ER			Presence of ER
	Total bound	Nonspecific bound	Specific bound	Total bound	Nonspecific bound	Specific bound
0.067	59	72		175	49	126
1.0	68	115		289	57	232
3.3	154	134	20	627	168	459
6.7	154	160		937	174	763
10.0	183	245	28	1,105	224	881
30.0	470	645		1,679	666	1,013

Values are the means of six to eight determinations at each concentration of [³H]estradiol. The yeast without the ER contained the empty 86 and 62 vectors. Nonspecific binding was determined in the presence of 1 μM unlabeled E2.