Figure 5.

Panx1 blockade inhibits ATP-mediated IL-1β release from activated macrophage. (A) IL-1β release from LPS-primed THP-1 macrophages after transfection with scrambled or panx1 siRNA70. Neither siRNA alone induced IL-1β release nor did the scrambled siRNA alter ATP-induced release relative to untransfected cells, but panx1 siRNA70 significantly inhibited ATP-induced release; results are representative of three similar experiments. ^**P<0.005. (B) IL-1β release from LPS-primed THP-1 macrophages under conditions indicated. CBX (20 μM), ¹⁰pan1 peptide (200 μM) and the monoclonal anti-P2X₇R Ab, but not the connexin blocking peptide gp27 (1 mM), significantly inhibited ATP-evoked IL-1β release. (C, D) Similar experiments performed on mouse J774 macrophage and human alveolar macrophage; ¹⁰panx1 inhibitory peptide completely blocked ATP-mediated IL-1β release from these cells. Results are representative of 3–6 similar experiments in each case; error bars are standard deviation of triplicate samples. ^**P<0.005.