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. 2006 Aug 4;91(10):3805–3818. doi: 10.1529/biophysj.106.082792

FIGURE 6.

FIGURE 6

Kinetics of YEHK21 melting and folding initiated with temperature change. (a) DUVRR spectra of YEHK21 measured 20, 60, and 340 s after the 25–85°C temperature jump. The spectra were measured in an open solution stream. The accumulation time was 20 s. (b and c) The difference spectra shown were obtained by subtracting DUVRR spectra of YEHK21 measured 20 and 340 s, respectively, after the temperature jump from that measured before the jump. (d) The difference between the room-temperature DUVRR spectra of YEHK21 obtained for the initial folded polypeptide and that briefly heated to 125°C and cooled to room temperature (see Fig. 5). (g) DUVRR spectra of YEHK21 measured at room temperature 1, 60, and 240 min after a 5-min exposure at 100°C. The spectra were measured in an NMR tube. The accumulation time was 60 s. The spectrum of the initial folded polypeptide (black) is shown for comparison. (e and f) The difference spectra shown were obtained by subtracting the DUVRR spectrum of the initial folded YEHK21 from those measured at room temperature 60 and 240 min, respectively, after a brief exposure at 100°C.