Figure 3.

Expression of AmA1 protein in pSB8 (A) and pSB8G (B) tubers of transgenic lines by immunoblot analysis. Aliquots of 50 and 100 μg of soluble protein from pSB8G and pSB8 tubers, respectively, were separated by SDS/12.5% PAGE and electroblotted onto Hybond-C membrane. AmA1 protein was detected with a polyclonal anti-AmA1 antibody and alkaline phosphatase-conjugated anti-rabbit IgG antibody. The alkaline phosphatase color development reaction for pSB8G blot (B) was carried out for 5 min, whereas the same reaction for pSB8 blot (A) was prolonged for 20 min. The lane numbers are representatives of the individual transgenic lines, whereas U represents untransformed tuber.