Table IV.
Primer and probe sequences used in the quantification of relative mRNA levels
| Gene | Type | Quantifying (5′ to 3′) |
|---|---|---|
| Amplicon: GA20ox1-104 | ||
| PsGA20ox1 | FPa | GCATTCCATTAGGCCAAATTTC |
| RPb | CCACTGCCCTATGTAAACAACTCTT | |
| Probe | CCTTCATGGCTCTTTC | |
| Amplicon: GA20ox2-88 | ||
| PsGA20ox2 | FP | AATACATCTTCTCTACCGTTGCAAAT |
| RP | TTGGCGGTGTTAAACAAGGTT | |
| Probe | ACATACCCTCAGAGTTC | |
| Amplicon: GA3ox1-87 | ||
| PsGA3ox1 | FP | TTCGAGAACTCTGGCCTCAAG |
| RP | ATGTTCCTGCTAACTTTTTCATGGTT | |
| Probe | ACAATATCACAGAATCTGGT | |
| Amplicon: GA2ox1-73 | ||
| PsGA2ox1 | FP | TTCCTCCTGATCATAGCTCCTTCT |
| RP | TTGAACCTCCCATTAGTCATAACCT | |
| Probe | GAGAATCACCAACATT | |
| Amplicon: GA2ox2-83 | ||
| PsGA2ox2 | FP | AACACAACAAAGCCTAGAATGTCAA |
| RP | ACCATCTTCGATAACGGGCTTAT | |
| Probe | TGTATTTTGCAGCACCACC | |
| Amplicon: 18S-62 | ||
| Ps18S rRNA | FP | ACGTCCCTGCCCTTTGTACA |
| RP | CACTTCACCGGACCATTCAAT | |
| Probe | ACCGCCCGTCGCTCCTACCG |
a
FP, Forward primer.
b
RP, Reverse primer