Figure 6.

Construction of WRKY7 overexpression lines. A, RNA-gel analysis of WRKY7 overexpression in the wild-type (35S:W7) or eds8 (eds8/35S:W7) background. RNA samples were prepared from leaves of 4.5-week-old wild-type (Wt), eds8, and transgenic plants and probed with a WRKY7 fragment. A representative transgenic WRKY7 line in the wild-type background and a representative transgenic WRKY7 line in the eds8 mutant background containing a single T-DNA insertion in their genomes and exhibiting stable WRKY7 expression were used in the analysis. Their F3 homozygous progeny plants were used in all the experiments in the study. B, Morphology of representative 5-week-old wild-type (Wt), eds8, and transgenic plants overexpressing WRKY7.