Table III.

Functional categorization of rhizome tissue-derived ESTs^a

Functional Categories	High RT/RMI	Low RT/RMI	High RT/AG	Low RT/AG	pSHR	Random Rhizomeb
No. of tested contigs and singlets	295c	56	242c	66	67	2,616
No homology	10.85 ± 3.55	8.93 ± 6.38	6.20 ± 3.04	10.61 ± 7.43	17.91 ± 5.16	2.91 ± 0.64
Unknown function	31.86 ± 5.32	30.36 ± 10.55	29.75 ± 5.76	30.30 ± 11.09	20.90 ± 4.83	44.65 ± 1.91
Miscellaneous	2.37 ± 1.74	1.79 ± 2.95	1.65 ± 1.61	1.52 ± 2.95	0.00 ± 0.00	2.29 ± 0.57
Primary metabolism	10.85 ± 3.55	8.93 ± 6.38	9.50 ± 3.70	12.12 ± 7.87	13.43 ± 4.38	12.35 ± 1.26
Secondary/hormone metabolism	4.41 ± 2.34	7.14 ± 5.76	11.16 ± 3.97	1.52 ± 2.95	5.97 ± 2.90	4.13 ± 0.76
Chromatin and DNA metabolism	1.69 ± 1.47	0.00 ± 0.00	2.48 ± 1.96	3.03 ± 4.14	2.99 ± 2.15	0.96 ± 0.37
Protein synthesis/processing	9.49 ± 3.34	26.79 ± 10.11	8.26 ± 3.47	7.58 ± 6.38	14.93 ± 4.67	10.24 ± 1.16
Gene expression/RNA metabolism	4.41 ± 2.34	1.79 ± 2.95	3.31 ± 2.25	3.03 ± 4.14	5.97 ± 3.02	3.10 ± 0.66
Transporter/trafficking	5.76 ± 2.66	8.93 ± 6.38	8.26 ± 3.47	9.09 ± 6.94	7.46 ± 3.30	7.00 ± 0.98
Signal transduction	4.07 ± 2.25	3.57 ± 4.14	3.72 ± 2.38	4.55 ± 5.03	2.99 ± 2.14	3.33 ± 0.69
Cell wall structure/metabolism	2.37 ± 1.74	0.00 ± 0.00	2.07 ± 1.79	1.52 ± 2.95	2.99 ± 2.15	1.72 ± 0.50
Cell division	0.68 ± 0.94	0.00 ± 0.00	0.41 ± 0.81	1.52 ± 2.95	1.49 ± 1.54	0.46 ± 0.26
Cytoskeleton	1.02 ± 1.14	0.00 ± 0.00	1.65 ± 1.61	0.00 ± 0.00	0.00 ± 0.00	1.68 ± 0.49
Defense and cell rescue	2.37 ± 1.74	0.00 ± 0.00	2.48 ± 1.96	3.03 ± 4.14	0.00 ± 0.00	1.26 ± 0.43
Abiotic stimuli/development	7.80 ± 3.06	1.79 ± 2.95	9.09 ± 3.62	10.61 ± 7.43	2.99 ± 2.08	3.94 ± 0.75

^aValues are percentages of unique sequences per treatment in each category ± approximate 95% confidence limits.

^bRandomly sampled EST-based contigs from rhizome cDNA libraries, i.e. S. halepense and S. propinquum.

^cEach of 71 clones with high RT/RMI as well as high RT/AG was assigned to both ratios.