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. 2006 Nov;142(3):1329–1339. doi: 10.1104/pp.106.086587

Figure 1.

Figure 1.

SDS-PAGE (14%). A, Detection of tomato JIP21. Crude protein extracts of untreated control tomato leaves (control); wounded tomato leaves harvested upon 48 h (wounding); and tomato leaves harvested after 48 h of a 2 mm MeJ treatment (MeJ). B, Purification of JIP21 protein. Crude, Crude extract of MeJ-treated tomato leaves; 20% to 35% AS, proteins present after 20% to 35% ammonium sulfate fractionating; SP-Seph I and II, enrichment achieved by two consecutive chromatographies on SP-Sephadex C25; MonoS, final FPLC step using a Mono S HR 5/5 column. Lane M, Mr standards. Proteins were stained with Coomassie Brilliant Blue R-250. Bands corresponding to JIP21 are indicated with an arrowhead.