Time-course analysis of JIP21 mRNA accumulation in tomato leaves, in response to wounding or MeJ treatment by northern blot. A, Local response to wounding. mRNAs from tomato wounded leaves, harvested 0, 1, 6, 12, 24, 48, and 72 h after wounding. B, Distal response to wounding. mRNAs from immediately upper tomato leaves, harvested 0, 1, 6, 12, 24, 48, and 72 h after wounding. C, Response to MeJ treatment. mRNAs from tomato leaves, harvested 0, 6, 12, 24, 36, 48, 72 h, 4 d, and 7 d after spraying plants with a 2 mm solution.