Figure 6.

Inhibitory activity of JIP21 on trypsin and chymotrypsin. Reactions containing 50 ng of trypsin (A) or chymotrypsin (B), and increasing amounts of JIP21 (continuous line), STI (dotted line), or BBI (dashed line) were preincubated in a final volume of 50 μL, and then 10 μL of fluoresceinated hemoglobin (0.5% [w/v]) were added as a substrate. The proteolytic activity was determined as soluble fluorescence measured at 525 nm. Enzymatic activity is expressed in relative terms as the net emitted fluorescence with respect to the control reaction. Three independent assays were performed for each protease experiment.