Figure 2.

Analysis of BnALMT expression in rape seedlings. A, Tissue-specific expression of BnALMT1 seedlings grown in complete nutrient solution. Plants were exposed to 0.5 mm CaCl₂ solution (pH 4.5) containing 0 or 50 μm Al for 6 h. RNA was isolated from the apical centimeter of the root (0–1 cm), the next centimeter of root (1–2 cm), and from young leaves. Semiquantitative RT-PCR was conducted on 5 μg total RNA as described in the “Materials and Methods.” β-tubulin was used as the reference gene. B, Effect of Al, La, Er, and Yb on BnALMT1 and BnALMT2 expression. Seedlings were exposed to 0.5 mm CaCl₂ (pH 4.5) containing 0 or 50 μm AlCl₃ in the presence or absence of 50 μm LaCl₃, ErCl₃, or YbCl₃ for 6 h. RNA was isolated from the roots and semiquantitative RT-PCR was conducted on 5 μg total RNA. β-tubulin was used as the reference gene (C is control with 0.5 mm CaCl₂).