Figure 5.

Expression of BnALMT1 and BnALMT2 in tobacco cultured cells. A, Construct inserted into the vector pBI121 and introduced into Agrobacterium tumefaciens strain LBA 4404 for transforming tobacco cells. B, BnALMT1 and BnALMT2 expression in the transgenic cells analyzed by RT-PCR using tobacco 18 S RNA as control. C, Effect of Al on malate efflux from two independent transgenic lines (1 and 2) expressing BnALMT1, BnALMT2, or the empty vector. The transgenic cells (100 mg fresh weight 10 mL⁻¹) were cultured in the dark condition at 25°C on a rotary shaker for 18 h in the Ca-Suc medium (pH 4.5) containing 0 or 100 μm AlCl₃. Malate efflux was determined from 10 mL culture as described in the “Materials and Methods.” Bars represent means ± se of three replicates and the experiment was repeated four times.