Figure 5.

Suppression of CmCCD4a expression by RNAi. A, Changes in carotenoid concentrations during petal development of wild-type (WT) and five independent CmCCD4a RNAi plants (1–5). Measurements were performed in triplicate, and mean values ± se are shown . E, Early; M, middle; L, late. B, Changes in CmCCD4a expression during petal development of wild-type and CmCCD4a RNAi plants. Quantitative real-time RT-PCR analyses were performed in triplicate using CmCCD4a-specific primers, and the expression levels were normalized against actin levels; mean values ± se are shown. C, Photographs of fully opened flowers of wild-type Sei-Marine and CmCCD4a RNAi plants (line 5).