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. Author manuscript; available in PMC: 2006 Nov 2.
Published in final edited form as: Oncogene. 2003 Jul 24;22(30):4664–4674. doi: 10.1038/sj.onc.1206619

Figure 2.

Figure 2

PRL transgene expression is enriched in mammary epithelial cells. (a) Total RNA isolated from mammary glands of 13-week-old mice from NRL-PRL lines 1655-8 and 1647-13, and nontransgenic (non-TG) littermates was examined for rPRL and GAPDH transcripts (positive internal control) using RT–PCR analysis as described in Materials and methods. (b) Total RNA from mammary glands that had been ‘cleared’ of epithelia from the two NRL-PRL lines and nontransgenic littermates (non-TG) was examined for rPRL, K18, and L19 transcripts. Intact mammary glands from a nontransgenic female (−) and 1655-8 littermate (+) serve as negative and positive controls, respectively. C denotes a control PCR reaction without RT. (c) Total RNA from multiple tissues of 13-week-old females (NRL-PRL 1655-8, 1647-13, nontransgenic littermates (non-TG)) were examined for rPRL transcripts. L19 transcripts serve as an internal control for RNA and loading. Intact mammary glands from a nontransgenic female (−) and 1655-8 littermate (+) serve as negative and positive controls, respectively