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Autophosphorylation of SOS2 kinase. GST, GST-SOS2, GST-SOS2 plus GST-SOS3, and mutated kinases GST-SOS2(K40N) and GST-SOS2(G197E) were expressed in E. coli, purified from bacterial lysates by means of glutathione-Sepharose chromatography, incubated with [γ-32P]ATP in kinase buffer, electrophoresed on SDS/polyacrylamide gel, and Coomassie stained (Left), and exposed to x-ray film (Right).
