Figure 6.

Regulation of Elip and Sep gene expression by stress. (A) Arabidopsis leaves were either directly used for isolation of total RNA (C, control) or exposed to light stress for 3 hr before RNA extraction (HL, high light). Northern blot hybridization was performed by using ³²P-labeled cDNA fragments of the Elip1, Elip2, Sep1, Sep2 clones and selected members of the Cab gene family, such as Lhca1, Lhcb3, and Lhcb6. The α-tubulin gene (clone ID 32C11T7) was hybridized as an internal control. (B) Arabidopsis leaves were exposed for 2 hr to various stress conditions: (1) control, (2) cold stress, (3) heat shock, (4) wounding, (5) salt stress, (6) desiccation, (7) oxidative stress, and (8) UV-A illumination. Isolated total RNA was used for Northern blot hybridization with the radioactively labeled inserts of the Elip and Sep cDNA clones. The rRNA pattern in the gel, visualized by staining with ethidium bromide, is shown in A (Right) and B (Lower).