Reduction in FLC transcript level caused by a 35S∷FLC antisense construct results in early-flowering in C24 and in the fca-1 mutant. (A) Flowering time of untransformed C24 and three C24 T2 lines (AS2, AS9, and AS12) containing the 35S∷FLC antisense construct, and of untransformed fca-1, three fca-1 T2 lines (AS1, AS5, and AS6), and Ler, expressed as rosette leaf number. Error bars represent the SE. (B) FLC expression in bolting, early-flowering T2 segregants and from bolting C24, fca-1, and Ler plants. The ethidium bromide-stained ribosomal bands are shown below the blots as loading controls.