Mutants with reduced responses to vernalization maintain a high FLC transcript level after cold treatment. (A) Flowering time expressed as rosette leaf number for Ler, fca-1, fca-1 vrn1–1, fca-1 vrn2–1, and vrn1–1, cold-treated for either 1 (black bars) or 28 (gray bars) days. Error bars represent the SE. (B) FLC expression in seedlings of Ler and the mutants in A, cold-treated for either 1 or 28 days, as indicated above the figure. The ethidium bromide-stained ribosomal bands are shown below the blots as loading controls.