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. 2000 Mar 14;97(7):3753–3758. doi: 10.1073/pnas.060023597

Figure 4.

Figure 4

The down-regulation of FLC and the decrease in flowering time is proportional to the duration of the cold treatment. (A) FLC expression and flowering time, expressed as rosette leaf number, in C24 seedlings that were subjected to a cold pretreatment of 1, 7, 14, 21, or 28 days, as indicated, before growth at 21°C. Error bars represent the SE in A and B, and the ethidium bromide-stained ribosomal bands are shown as loading controls in A–C. (B) FLC expression and flowering time, expressed as rosette leaf number, in Ptz-1 seedlings that were subjected to a cold pretreatment of 1, 14, 28, or 56 days, as indicated, before growth at 21°C. (C) FLC expression in flc-11 seedlings that were subjected to a cold pretreatment of 1, 28, or 56 days, as indicated, before growth at 21°C. (D) FLC protein levels in the soluble fraction from Ptz-1, flc-11, C24, Ler, and flc-13 seedlings, cold-treated for either 2 or 28 days, as indicated. The amido black-stained filter is shown below as a loading control. The migration of the molecular weight markers (×1000) is shown to the left. A band detectable with the FLC antibody, but not with the preimmune serum, is indicated as FLC.