Figure 5.

The down-regulation of FLC transcript by cold treatment is mitotically stable, but is reset in progeny. (A) FLC expression in 28-day cold-treated C24, harvested after 1, 2, 5, 12, or 19 days of growth at 21°C, as indicated above the figure. The majority of the plants were bolting at 19 days. (B) FLC expression in tissues of 1- and 28-day cold-treated C24 plants. Root and aerial tissue were from one set of seedlings, and floral bolts (2–3 cm in height) and rosette leaves were derived from another set of plants. (C) FLC expression in C24 seedlings that were the progeny of plants that were either cold-treated for 28 days (lane 1) or for 1 day (lanes 2 and 3), which, in turn, themselves were either cold-treated for 1 day (lanes 1 and 2) or for 28 days (lane 3). The ethidium bromide-stained ribosomal bands are shown below the blots as loading controls.