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(A) SLR1 affinity column chromatography. Chromatogram monitored at 280 nm (Upper). SLR1-binding activity measured by BIAcore (Lower). The arrow indicates the position at which the elution started. (B) Reverse-phase HPLC (on VP-318 column) purification of the SLR1-binding fractions. The dotted line shows the concentration of acetonitrile. Hatched bars indicate SLR1-binding activity. SLR1-BP1 (1) and SLR1-BP2 (2) were eluted at 22.2 min and 22.7 min, respectively.
