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. 2000 Mar 14;97(7):3765–3770. doi: 10.1073/pnas.040580797

Figure 5.

Figure 5

(A) Tissue-specific expression of SLR1-BP. An RNA gel blot containing total RNA from anther, stigma, and leaf tissues was hybridized with the SLR1-BP coding region probe (Upper). Numbers 1–8 represent different bud sizes, with 1 = 0–1 mm, 2 = 1–2 mm, 3 = 2–3 mm, 4 = 3–4 mm, 5 = 4–5 mm, 6 = 5–7 mm, 7 = 7–10 mm, and 8 = open flower. The RNA gel was stained with ethidium bromide before blotting (Lower). (B) In situ hybridization of SLR1-BP. Anther sections derived from 5- to 7-mm-long flower buds were hybridized with an SLR1-BP antisense riboprobe and an SLR1-BP sense riboprobe (negative control). (C) Distribution analysis of SLR1-BP. An RNA gel blot containing total anther RNA from B. campestris (AA, 2n = 20; lane 1), B. napus (AACC, 2n = 38; lane 2), B. oleracea (CC, 2n = 18; lanes 3 and 4), B. juncea (AABB, 2n = 36; lane 5), B. nigra (BB, 2n = 16; lane 6), R. sativus (lane 7), and Arabidopsis thaliana (lane 8) was hybridized with the SLR1-BP coding region probe (Upper). The RNA gel was stained with ethidium bromide before blotting (Lower). (D) Alignment of the deduced amino acid sequences of SLR1-BP from B. campestris (BcSLR1-BP), B. juncea (BjSLR1-BP), and B. napus (BnSLR1-BP). Conserved amino acid residues are indicated by asterisks.