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. 1999 Apr 13;96(8):4262–4267. doi: 10.1073/pnas.96.8.4262

Figure 1.

Figure 1

Characteristics of DNA electrotransfer in muscle fibers. Bars represent the mean ± SEM of individual values (triangles), observed in the absence (white bars) or the presence (gray bars) of electric-pulse delivery. The ordinate scale is logarithmic. (A) Influence of the applied voltage-to-distance ratio (eight pulses; 20 ms per pulse; 1 Hz). (B) Influence of pulse length (eight pulses; 200 V/cm; 1 Hz). (C) Influence of pulse number (200 V/cm; 20 ms per pulse; 1 Hz). (D) Influence of the frequency of pulse delivery (eight pulses; 200 V/cm; 20 ms per pulse). (E) Dependence of luciferase gene expression on the amount of injected DNA in a constant volume of 30 μl (eight pulses; 200 V/cm; 20 ms per pulse; 1 Hz); (F) Kinetics of luciferase gene expression in the absence (open circles) or presence (filled squares) of electrotransfer (eight pulses; 200 V/cm; 20 ms per pulse; 2 Hz). Abscissa: time after plasmid injection. Mice were killed 7 days (A–E) or at the indicated times (F) after DNA transfer.