Figure 1.
Trypsin protection of [35S]methionine-labeled in vitro translates of αs-WT (Upper) and αs-Q170A (Lower). In each gel, the full-length undigested αs (52 kDa) is shown in the first lane, and complete digestion in the absence of activators is shown in the second lane. The next four lanes show the results after preincubation at the temperatures and in the presence of various agents indicated below. The smaller products in the left lane are attributable to initiation of protein synthesis at downstream methionine codons. Gs, stimulatory G protein.