Table 1.
Effect of adenosine analogues on trypansomatid GAPDH (IC50) and on growth of cultured parasites (ED50)
| Compound | L. mexicana, | T. brucei, | T. cruzi, | 3T3 fibroblasts,* | ||
|---|---|---|---|---|---|---|
| IC50 | IC50† | ED50‡ | IC50 | ED50 | ED50 | |
| Adenosine | 5 × 104 | 105 | >50 | 105 | >50 | >50 |
| 2 | >100 | >50 | 20 | >50 | ||
| 3 | 150 | >50 | >50 | >50 | ||
| 4 | 80 | >50 | >50 | >50 | ||
| 5 | 0.28 | 2 | 30 | 5 | 12 | >50 |
| 6 | 83 | >1,000 | >50 | >1,000 | >50 | >50 |
| 7 | 5 | 25 | 50 | 45 | 20 | >50 |
| 8 | 10 | 30 | 15 | 14 | 3 | 45 |
All values are in μM.
*
Noninfected host cells used to support growth of T. cruzi amastigotes.
†
IC50 values were determined by measuring GAPDH inhibition at 3–5 concentrations (10% error) (29).
‡
Statistical error limits on the ED50 values determined in triplicate are 15% (20% for separate experiments).