Figure 6.

In situ analysis of dronc mRNA expression during Drosophila development. Expression of dronc transcript was detected by in situ hybridization with a digoxigenin-labeled antisense mRNA probe. (A) A stage 3 syncitial embyro showing high levels of dronc expression. (B) A stage 8 embryo with uniform dronc expression throughout. (C) By stage 13 dronc expression is reduced throughout. Arrow indicates amnioserosa. (D) A stage 13 embryo hybridized with the dronc sense control probe shows no background staining. (E) A late third instar larval midgut with high dronc expression. (F) A late third instar larval midgut hybridised with the sense control riboprobe. (G) A late third instar larval salivary gland showing high dronc expression. (H) dronc sense control on late third instar salivary gland. (I) A late second instar midgut showing low levels of dronc expression. (J) A second instar salivary gland tissue showing no dronc expression. (K) A late third instar eye imaginal disc shows ubiquitous low level of dronc mRNA and higher staining in a subset of blood cells associated with the eye disc (arrow). (L) dronc sense control on late third instar eye imaginal disc. (M) Brain lobes from late second instar larva showing ubiquitous low levels of dronc mRNA. (N) dronc sense control on late second instar brain lobes shows weak background staining. (O) Adult egg chambers showing high expression of dronc particularly at the later stages of oogenesis. The stages of oogenesis for each chamber are indicated. (P) dronc sense riboprobe control on adult egg chambers.