The sequence of human FAT10 gene. (A) The human FAT10 gene contains 971 base pairs spanning two exons. There are three short uORFs (starting at nucleotides 21, 82, and 176) preceding the long FAT10 ORF (225 to 722). Potential translation initiation codons for the short uORF are indicated by lower-case bold letters and that for FAT10 by upper-case bold letters. The FAT10 coding sequence represented by capital letters encodes a 166-aa polypeptide. The four-kilobase intron is located between codons 9 and 10, represented by bold letters. The poly(A) signal of AATAAA is underlined at the 3′ end of FAT10 gene. The access number for this sequence is AF123050. (B) Comparison of human, murine, partial rat, and porcine FAT10 proteins with classical ubiquitin amino acids sequences. The ubiquitin sequence is shown twice to compare with the two FAT10 UBL domains, and only the conserved amino acids are presented in the diagram.