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BglG and RNAP can be coimmunoprecipitated from cellular extracts. Extracts of cells enriched for [35S]BglG were prepared as described in Material and Methods. The major labeled product was BglG (lane M). The extracts were incubated either without (lane 1) or with (lane 2) antibodies against RNAP. Samples were analyzed by using SDS/PAGE followed by autoradiography. Molecular masses of protein standards are given in kDa.
