Figure 4.

BglG interacts with the N-terminal region of β′ subunit. (A) Extracts of cells overproducing β′ (1) or one of the truncated β′ proteins, β′-(1–517 and 1266–1407) (2), β′-(1–876) (3), β′-(545–1407) (4), and β′-(877–1407) (5), were fractionated by using SDS/PAGE, blotted onto a nitrocellulose filter, and probed with MBP–BglG and then with antibodies against MBP (A) or stained with Coomassie blue (B). Arrowheads indicate the positions of β′ and its derivatives. (C) Schematic representation of β′ and its derivatives. Numbers of amino acids are indicated. Boxed areas A to H indicate evolutionarily conserved regions (56). + indicates binding of BglG to the β′ derivative; − indicates lack of binding.